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nencki.gov.pl » Working Groups » Department of Molecular and Cellular Neurobiology »
Laboratory of Calcium Binding Proteins

Head
Anna FILIPEK

Staff
Jacek KUŹNICKI, Ewa KILAŃCZYK (post-doc), Wiesława LEŚNIAK, Wojciech MICHOWSKI (PhD student), Wiktor PRUS (PhD student), Gabriela SCHNEIDER (PhD student), Łukasz SŁOMNICKI (PhD student), Magdalena ZABKA (PhD student), Agnieszka GRACZYK (MSc student), Barbara ZARZYCKA (technician)

Research profile

The laboratory is focused on studying the signal transduction processes leading to cell proliferation and differentiation; in particular we concentrate on a calcium binding protein – S100A6 and its targets such as Sgt1, CacyBP/SIP and p53. We study the involvement of these proteins in protein ubiquitination, in cell response to stress factors and in cytoskeleton rearrangement under normal and pathological conditions. We also study the  transcriptional and epigenetic regulation of genes encoding S100A6, Sgt1 and CacyBP/SIP, as well as posttranslational modifications of these proteins in relation to their function.

Current research activities

  • Studies of the expression and function of Sgt1 and CacyBP/SIP in cells under stress conditions (heat shock or heavy metals) by means of western blot, immunocytochemistry and mass spectrometry.
  • Studies of the role of S100A6 and CacyBP/SIP in protein ubiquitination using cells overexpressing or deficient in S100A6 and CacyBP/SIP due to transfection with appropriate plasmids.
  • Studies of the involvement of CacyBP/SIP in rearrangement of neuronal cytoskeleton, studies of the binding of CacyBP/SIP to tubulin and to other cytoskeletal proteins by means of chemical cross-linking, co-immunoprecipitation, immunocytochemistry etc.
  • Studies of the impact of S100A6 deficiency on various aspects of cell physiology using cells stably transfected with siRNA against S100A6.
  • Studies of the transcriptional and epigenetic regulation of gene expression by means of promoter activity assays, gel-shift technique, chromatin immunoprecipitation (ChIP) and bisulfite DNA modification.

Selected publications

Spiechowicz M, Zylicz A, Bieganowski P, Kuznicki J, Filipek A (2007) Hsp70 is a new target of Sgt1-an interaction modulated by S100A6. Biochem Biophys Res Commun., 357: 1148-53.

Lesniak W, Slomnicki LP, Kuznicki J (2007) Epigenetic control of the S100A6 (Calcyclin) gene expression. J Invest Dermatol., 127: 2307-14.

Schneider G, Nieznanski K, Kilanczyk E, Bieganowski P, Kuznicki J, Filipek A, (2007) CacyBP/SIP interacts with tubulin in neuroblastoma NB2a cells and induces formation of globular tubulin assemblies. Biochim Biophys Acta – Molecular Cell Resarch., 1773: 1628-36

Spiechowicz M, Bernstein H-G, Dobrowolny H, Leśniak W, Mawrin Ch, Bogerts B, Kuźnicki J, Filipek A (2006) Density of Sgt1- immunopositive neurons is decreased in the cerebral cortex of Alzheimer’s disease brain. Neurochem Int., 49: 487-493

Leśniak W, Kuźnicki J. (2006) Binding and functional characteristics of two E-box motifs within the S100A6 (calcyclin) gene promoter. J Cell Biochem 97: 1017-1024



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Published at: 2007-11-19 11:42
 

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