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nencki.gov.pl » Working Groups » Department of Biochemistry »
Laboratory of Cell Signaling and Metabolic Disorders

  Head
Agnieszka DOBRZYŃ

Staff
Paweł DOBRZYŃ, Magdalena JAZUREK (PhD student), Konrad SZYMAŃSKI (Ph D Student), Aleksandra Pypeć (technician)

Research profile

The laboratory is focused on the molecular mechanisms of lipotoxicity with special emphasis to lipid induced insulin resistance and the role of fatty acids in the regulation of gene expression. We are especially interested in understanding the interrelationships between obesity and its most common complications, e.g. diabetes and heart disease. The focus of our studies is the signaling and transcriptional cascades that have far-reaching implications on lipid metabolism and regulation of human metabolic diseases.

We are using primary cell culture and animal models to reveal new biochemical mechanisms underlying the key features of obesity and diabetes. This involves molecular biology and biochemical studies including gene expression and enzyme activity assays, protein and lipid analysis, chromatography (TLC, GC, HPLC) and many other standard biochemical methods.

 

Current research activities

  • determining molecular mechanisms involved in the pathogenesis of lipid-induced insulin resistance - focus on the role of AMPK and ceramide pathways;  
  • studies of transcription factors (STAT, PPAR, PGC1, SREBP) as key molecules involved in lipotoxicity; 
  • determining the role of stearoyl-CoA desaturase in pathogenesis of lipotoxic heart disease;
  • determining molecular mechanisms of lipid-induced apoptosis in β cells.

Selected publications

Sampath H., Miyazaki M., Dobrzyn A., Ntambi J.M. (2007) Stearoyl-CoA desaturase-1 mediates the pro-lipogenic effects of dietary saturated fat. J. Biol. Chem. 282:2483-93.

Dobrzyn A., Dobrzyn P. (2006)  Stearoyl-Co A desaturase – a new player in skeletal muscle metabolism regulation. J. Physiol. Pharmacol. 57: 31-42.

Dobrzyn A., Dobrzyn P., Miyazaki M., Sampath H., Chu K., Ntambi J.M. (2005) Stearoyl-CoA desaturase 1 deficiency increases CTP: Choline cytidylyltransferase translocation into the membrane and enhances phosphatidylcholine synthesis in liver. J. Biol. Chem. 280: 23356-23362.

Dobrzyn A., Dobrzyn P., Lee SH, Miyazaki M., Cohen P., Asilmaz E., Hardie D.G., Friedman J.M., Ntambi J.M. (2005) Stearoyl-CoA desaturase 1 deficiency reduces ceramide synthesis by down-regulating serine palmitoyltransferase and increasing b-oxidation in skeletal muscle. Am. J. Physiol. Endocrinol. Metab. 288: E599-E607.

Dobrzyn P., Dobrzyn A., Miyazaki M., Cohen P., Asilmaz E., Hardie D.G., Friedman J.M., Ntambi J.M. (2004) Stearoyl-CoA desaturase 1 deficiency increases fatty acid oxidation by activating AMP-activated protein kinase in liver. Proc. Natl. Acad. Sci. USA 101: 6409-6414.



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Published at: 2007-11-20 11:59
 

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