Staff Jolanta JÓŹWIAK, Krzysztof NIEZNAŃSKI,
Paweł POMORSKI, Dariusz STĘPKOWSKI,
Magdalena SOBCZAK, Emilia KARCZEWSKA(technician),
Serhiy HAVRYLOV(PhD student),
Justyna KAROLCZAK (PhD student), Łukasz MAJEWSKI(PhD student),
Katarzyna M. OSIECKA(PhD student), Dorota WYPYCH (PhD student)
involvement of unconventional myosins, cytoskeletal and
adaptor proteins in cell locomotion and intracellular
trafficking; structure-function relationships in myosin and
actin, the key proteins involved in force generation and
motility in both muscle and non-muscle cells;
characterization of heterogeneity of prion protein complexes
and functional implications of their interaction with
microtubules; plasticity of contractile apparatus from
techniques: protein chemistry, biochemistry, fluorescence spectroscopy,
molecular biology, cell biology (including micromanipulations and in vivo
observations); fluorescence and electron microscopy.
Current research activities
investigation of involvement of unconventional myosins (in
particular of class VI) in cell locomotion and intracellular
trafficking of organelles and particles; studies are
performed on normal and transformed mammalian and amoebae
analysis of subcellular localization and inter-protein
interactions of Ruk/CIN85 adaptor protein;
investigation of the function of a novel actin-binding
protein (ApABP-FI) in Amoeba proteus;
probing the interaction of prion protein with microtubular
system; elucidation of the mechanism of translocation of
prion proteins within the cell;
investigation of actin filament dynamics; in particular,
effects of ionic strength, the kind of tightly bound
divalent cation, and nucleotide n the process of actin
polymerization are studied;
determining the actin-binding interface on skeletal muscle
myosin subfragment 1 in force generating state;
analysis of contractile apparatus in normal and denervated
muscle subjected to physical training.
Jakubiec-Puka A., Sławińska U., Rędowicz M.J., Biral D.,
Łapińska I., Chomontowska H., Krawczyk K., Bilski H.,
Karczewska E., Pliszka B. (2008) Influence of locomotor
training on the structure and myosin heavy chains of the
denervated rat soleus muscle. Neurological
Research, 30, 170-178.
M.J. Rędowicz (2007) Unconventional myosins in muscle.
Eur. J. Cell Biol., 86, 549-558.
M. Sobczak, E. Kocik, M.J. Rędowicz (2007) Novel Actin-binding
Filamin Immunoanalog with a Coiled-Coil Region in
Amoeba proteus. Biochem. Cell Biol., 85,
K. Nieznański, Z.A. Podlubnaya, H. Nieznańska (2006)
Prion protein inhibits microtubule assembly by inducing
tubulin oligomerization. Biochem. Biophys. Res. Commun.
B. Wawro,S.Yu. Khaitlina, A. Galińska-Rakoczy,
H. Strzelecka-Gołaszewska (2005) Role of actin DNase-I-binding
loop in myosin subfragment 1-induced polymerization of
G-actin. Implications to the polymerization mechanism.
Biophys. J.88, 2883-2896