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Laboratory of Motor Proteins
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nencki.gov.pl » Working Groups » Department of Biochemistry »
Laboratory of Motor Proteins

Head
Andrzej A. KASPRZAK

Staff
Elżbieta KOCIK (PhD student), Seweryn BAJER (PhD student), Ewa SZCZĘSNA (technician)

Research profile

mechanism of force generation by motor proteins; common structural elements of the motors; kinesin Ncd, a microtubule minus-end directed motor; interactions between motor domains in Ncd; fluorescence resonance energy transfer (FRET); in vitro motility measurements of motor proteins; total internal reflection fluorescence (TIRF) microscopy; the role of motor proteins in cell division; mechanism of Ncd transport by the end-tracking protein EB1.

Current research activities

  • Is the ability of the mitotic kinesin Ncd to recognize the relative orientation of transported microtubules associated with the subunit structure of the motor?

  • Reconstruction of the microtubule plus-end tracking system of Ncd by EB1. Observation of the transport in vitro using a TIRF microscope.

  • Mapping of the interacting regions on the Ncd-EB1 complex using point mutations in the binding areas.

  • Identification of an Ncd domain responsible for localisation of the motor in the spindle at various stages of cell division. An in vivo study using Drosophila S2 cells.

Selected publications

Fink G., Hajdo Ł., Skowronek K. J., Reuther C., Kasprzak A. A., Diez  S. (2009). The mitotic kinesin-14 Ncd drives directional microtubule-microtubule sliding. Nat. Cell Biol., 11:717-723

Kocik E., Skowronek K.J., Kasprzak A. A. (2009). Interactions between subunits in heterodimeric Ncd molecules.  J. Biol. Chem. 284: 35735-35745

Skowronek K.J., Kocik E., Kasprzak A. A. (2007). Subunits interactions in dimeric kinesins. Eur. J. Cell Biol. 86:559-568

Kasprzak A. A. (2007) The use of FRET in the analysis of motor protein structure. In  Methods in Molecular Biology, 392: 183-197

Reuther C., Hajdo Ł., Tucker R., Kasprzak, A. A., Diez, S. (2006). Biotemplated nanopatterning of planar surfaces with molecular motors. Nano Lett. 6: 2177-2183



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Published at: 2010-05-12 11:18
 

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