Home
About Institute
Working Groups
Department of Biochemistry
Laboratory of Biochemistry of Lipids
Laboratory of Bioenergetics and Biomembranes
Laboratory of Cell Signaling and Metabolic Disorders
Laboratory of Cellular Metabolism
Laboratory of Comparative Enzymology
Laboratory of Intracellular Ion Channels
Laboratory of Molecular Bases of Aging
Laboratory of Molecular Basis of Cell Motilty
Laboratory of Motor Proteins
Education
European Grants
Press Release
Library
Calendar of Events
Archives
Links
Visitor Information
Contact
nencki.gov.pl » Working Groups » Department of Biochemistry »
Laboratory of Molecular Bases of Aging

Head
Ewa SIKORA

Staff
Anna BIELAK-ŻMIJEWSKA, Magdalena DUDKOWSKA, Barbara GRZELAKOWSKA-SZTABERT, Grażyna MOSIENIAK, Katarzyna PIWOCKA, Małgorzata ŚLIWIŃSKA (PhD student), Kamila WOLANIN (PhD student), Monika KUSIO (PhD student), Zbigniew KORWEK (PhD student), Aneta BABIK, Dorota JANISZEWSKA  

Research profile

There is evidence of overlapping issues in the biology of aging and the biology of cancer on organismal as well as cellular levels. The major interest is biogerontology and biology of cancer focused on the understanding the molecular mechanisms regulating cell proliferation, cellular senescence and cell death.

Current research activities

  • Studies on the immunosenescence focused on searching for immunological markers (T cell phenotypes, propensity to undergo apoptosis and proliferation capacity) possibly determining healthy aging and longevity.
  • Searching for the longevity genes in the frame of FP6 GEHA-genetics for healthy aging coordinated by Prof. Claudio Franceschi;
  • Studies on cellular senescence in vivo and in vitro elucidating mechanisms of cell cycle regulation and proliferation cessation, telomere erosion, functional activities and cell capacity to undergo apoptosis;
  • Investigation of molecular mechanisms leading to cancer senescence induced by low doses of DNA damaging agents and Curcumin, a natural polyphenol derived from Curcuma longa; cancer cell senescence as a potential anticancer strategy;
  • Investigation of the Bcr-Abl-dependent pro-survival and antiapoptotic pathways with particular interest in the role of p53, GSK-3beta kinase, Bcl-2 family proteins and endoplasmic reticulum stress. Studies on mitosis and spindle assembly checkpoint regulation in Bcr-Abl expressing cells; the possible connection with genomic instability in leukaemic cells;
  • Studies on apoptosis resistance of cancer cells and mechanisms of cell death types alternative to apoptosis with particular interest in mitotic catastrophe; Curcumin, as a potent inducer of various types of cell death;
  • Studies on activation of Akt/PKB and ERK ½ kinases and their possible participation in down regulation of ornithine decarboxylase expression induced via cell membrane receptor c-Met and androgen receptor in mouse kidney;

Selected publications

Sliwinska M.A,. Mosieniak G., Wolanin K., Babik A., Piwocka K., Magalska A., Szczepanowska J., Fronk J and  Sikora E.  (2008) Induction of senescence with doxorubicin leads to increased genomic instability of HCT116 cells. Mech  Ageing Dev doi:10.1016/j.mad.2008.04.011

Franceschi C, Bezrukov V, Blanché H, Bolund L, Christensen K, de Benedictis G, Deiana L, Gonos E, Hervonen A, Yang H, Jeune B, Kirkwood TB, Kristensen P, Leon A, Pelicci PG, Peltonen L, Poulain M, Rea IM, Remacle J, Robine JM, Schreiber S, Sikora E, Slagboom PE, Spazzafumo L, Stazi MA, Toussaint O, Vaupel JW. (2007) Genetics of healthy aging in Europe: the EU-integrated project GEHA (GEnetics of Healthy Aging). Ann N Y Acad
Sci
. 2007;1100:21-45.

Mosieniak G., Sliwinska M., Piwocka K., Sikora E. (2006) Curcumin abolishes apoptosis resistance of calcitriol-differentiated
HL-60 cells. FEBS Lett. 580(19): 4653-60

Wolanin K., Magalska A., Mosieniak G., Klinger R., McKenna S., Vejda S., Sikora E., Piwocka K. (2006) Curcumin affects components of the chromosomal passenger complex and induces mitotic catastrophe in apoptosis-resistant Bcr-Abl-expressing cells. Mol Cancer Res. 4(7): 457-69

Sikora E., Bielak-Zmijewska A., Magalska A., Piwocka K., Mosieniak G., Kalinowska M., Widlak P., Cymerman IA., Bujnicki JM. (2006) Curcumin induces caspase-3-dependent apoptotic pathway but inhibits DNA fragmentation factor 40/caspase-activated DNase endonuclease in human Jurkat cells. Mol Cancer Ther. 5(4): 927-34



top of the page

Published at: 2008-05-27 9:44
 

Search WWW Search this site