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nencki.gov.pl » Working Groups » Department of Biochemistry »
Laboratory of Intracellular Ion Channels

Head
Adam SZEWCZYK

Staff
Antonina CHMURA-SKIRLIŃSKA, Marta GŁĄB (PhD student), Izabela KOSZELA-PIOTROWSKA, Bogusz KULAWIAK (PhD student), Dominika MALIŃSKA (PhD student), Antoni WRZOSEK

Research profile

Intracellular ion channels, intracellular ion homeostasis, pharmacology of intracellular potassium channels, pharmacology of mitochondria, interaction of potassium channel openers with mitochondria, role of mitochondria in ischemic preconditioning and cytoprotection, intracellular receptors for antidiabetic sulfonylureas and potassium channel openers, role of mitochondrial ion channels in apoptosis, regulation of chromaffin granules potassium channels by pH and nucleotides. More information about Laboratory on the web page

Current research activities

  • interaction of potassium channel openers such as diazoxide with mitochondrial ATP-regulated potassium channels;
  • regulation of mitochondrial ATP-regulated potassium channels by phosphorylation;
  • influence of potassium channels openers such as NS1619 and CGS7184 on human glioma cell line LN229 mitochondria;
  • mechanism of ischemic preconditioning and neuroprotection induced by diazoxide and other potassium channel openers;
  • regulation, measured by 86Rb+ flux and single channel activity, of chromaffin granules potassium channel by ATP and pH;
  • interactions of potassium channel effectors (channel inhibitors and activators) with skeletal muscle mitochondria and skeletal muscle cells such as L6 and C2C12 cell lines;
  • regulation of mitochondrial megachannel by antidiabetic sulfonylureas such as glibenclamide;
  • cytoprotective action of potassium channel openers suck as diazoxide in skeletal muscle mitochondria;
  • regulation of calcium homeostasis by potassium channel openers in skeletal muscle cells;
  • reconstitution of intracellular ion channels into planar lipid bilayers;
  • effects of potassium channel openers on imortalized mouse hippocampal cell line HT-22.

Selected publications

Kicinska A., Swida A., Bednarczyk P., Koszela-Piotrowska I., Choma K., Dolowy K, Szewczyk A, Jarmuszkiewicz W. (2007) ATP-sensitive potassium channel in mitochondria of the eukaryotic microorganism Acanthamoeba castellanii. J. Biol. Chem. 282: 17433-17441.

Szewczyk A., Skalska J., Głab M., Kulawiak B., Malińska D., Koszela-Piotrowska I., Kunz W.S. (2006) Mitochondrial potassium channels: from pharmacology to function. Biochim. Biophys. Acta. 1757: 715-720.

Skalska J., Debska G., Kunz W.S., Szewczyk A. (2005)Antidiabetic sulphonylureas activate mitochondrial permeability transition in rat skeletal muscle. Br. J. Pharmacol. 145: 785-91.

Bednarczyk P., Kicinska A., Kominkova V., Ondrias K., Dolowy K., Szewczyk A. (2004) Quinine Inhibits Mitochondrial ATP-regulated Potassium Channel from Bovine Heart. J. Membr. Biol. 199: 63-72

Szewczyk A., Wojtczak L. (2002) Mitochondria as a pharmacological target. Pharmacol. Rev. 54: 101-27



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Published at: 2007-11-15 12:25
 

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